GAPVD1 抗体 (N-Term)
Quick Overview for GAPVD1 抗体 (N-Term) (ABIN630695)
抗原
See all GAPVD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- GAPVD1 antibody was raised against the N terminal of GAPVD1
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纯化方法
- Affinity purified
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免疫原
- GAPVD1 antibody was raised using the N terminal of GAPVD1 corresponding to a region with amino acids FKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDPNKLIERFSPSQQ
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anti-GTPase Activating Protein and VPS9 Domains 1 (GAPVD1) (AA 1-629) antibody
GAPVD1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-GTPase Activating Protein and VPS9 Domains 1 (GAPVD1) (AA 182-231) antibodyGAPVD1 适用: 人, 小鼠, 犬, 大鼠, Cow, 豚鼠, 马, 兔, Bat, 小鸡, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GTPase Activating Protein and VPS9 Domains 1 (GAPVD1) (AA 1-260) antibodyGAPVD1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase Activating Protein and VPS9 Domains 1 (GAPVD1) (N-Term) antibodyGAPVD1 适用: 人, 小鼠, 犬, 大鼠, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GTPase Activating Protein and VPS9 Domains 1 (GAPVD1) antibodyGAPVD1 适用: 小鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 0.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator. -
说明
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GAPVD1 Blocking Peptide, catalog no. 33R-2950, is also available for use as a blocking control in assays to test for specificity of this GAPVD1 antibody
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of GAPVD1 antibody in PBS
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浓度
- Lot specific
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缓冲液
- PBS
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- GAPVD1 (GTPase Activating Protein and VPS9 Domains 1 (GAPVD1))
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别名
- GAPVD1
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背景
- GAPVD1 acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. It acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. It is also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. It has GEF activity for Rab5 and GAP activity for Ras.
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分子量
- 166 kDa (MW of target protein)
抗原
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