Golgi Complex 抗体
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- 抗原
- Golgi Complex
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 原理
- Mouse anti-Human Golgi Complex Antibody [Sodium Azide Free]
- 特异性
- Golgi complex in cytoplasm
- 纯化方法
- SU-DHL-1 large cell lymphoma cells were used as the immunogen.
- 免疫原
- SU-DHL-1 large cell lymphoma cells were used as the immunogen.
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- 应用备注
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Western Blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL,
Immunocytochemistry (Acetone/paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 缓冲液
- In 1X PBS ( pH 7.4), BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- 抗原
- Golgi Complex
- 背景
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Target Description: Clone 371-4 antibody specifically detects the Golgi complex in human cells. It is part of panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. The antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. The 371-4 antibody is an excellent marker for human cells in xenographic model research.
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