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ICAM-3/CD50 抗体

The 小鼠 单克隆 anti-ICAM-3/CD50 antibody is suitable to detect ICAM-3/CD50 in samples from 人. It has been validated for FACS, IF, IHC, Func 和 BP.
产品编号 ABIN6297704
发货至: 中国
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Quick Overview for ICAM-3/CD50 抗体 (ABIN6297704)

抗原

See all ICAM-3/CD50 (ICAM3) 抗体
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This ICAM-3/CD50 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Functional Studies (Func), Blocking Peptide (BP)
  • 原理

    Mouse anti-Human CD50 Antibody [Sodium Azide Free]

    特异性

    Cell surface

    纯化方法

    Stimulated human leukocytes were used as the immunogen for this CD50 antibody.

    免疫原

    Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
  • 应用备注

    Functional Activity (Blocking): Blocks CD50-CD11a interaction
    FACS: 0.5-1 μg/million cells
    IF: 0.5-1 μg/mL
    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS ( pH 7.4), BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    别名

    Intercellular adhesion molecule 3

    背景

    Target Description: This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.

    Gene Symbol: ICAM3

    基因ID

    3385

    UniProt

    P32942
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