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ICAM-3/CD50 抗体

This anti-ICAM-3/CD50 antibody is a 小鼠 单克隆 antibody detecting ICAM-3/CD50 in FACS, IF 和 IHC. Suitable for 人.
产品编号 ABIN6297692
发货至: 中国

Quick Overview for ICAM-3/CD50 抗体 (ABIN6297692)

抗原

See all ICAM-3/CD50 (ICAM3) 抗体
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

适用

  • 154
  • 3
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宿主

  • 100
  • 53
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小鼠

克隆类型

  • 116
  • 38
单克隆

标记

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This ICAM-3/CD50 antibody is un-conjugated

应用范围

  • 91
  • 49
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  • 40
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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
  • 原理

    Mouse anti-Human ICAM-3 Antibody [Sodium Azide Free]

    特异性

    Cell surface

    纯化方法

    Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.

    免疫原

    Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    别名

    Intercellular adhesion molecule 3

    背景

    Target Description: Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.

    Gene Symbol: ICAM3

    基因ID

    3385

    UniProt

    P32942
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