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CD43 抗体

This anti-CD43 antibody is a 小鼠 单克隆 antibody detecting CD43 in FACS, WB, IHC 和 IF. Suitable for 人.
产品编号 ABIN6297682
发货至: 中国

Quick Overview for CD43 抗体 (ABIN6297682)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 原理

    Mouse anti-Human CD43 Antibody [Sodium Azide Free]

    特异性

    Cell surface

    纯化方法

    Stimulated human leukocytes were used as the immunogen for the CD43 antibody.

    免疫原

    Stimulated human leukocytes were used as the immunogen for the CD43 antibody.
  • 应用备注

    Western blot: 1-2 μg/mL
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    Leukosialin

    背景

    Target Description: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

    Gene Symbol: SPN///CD43

    基因ID

    6693

    UniProt

    P16150

    途径

    Regulation of Leukocyte Mediated Immunity
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