CD43 抗体
Quick Overview for CD43 抗体 (ABIN6297682)
抗原
See all CD43 (SPN) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human CD43 Antibody [Sodium Azide Free]
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特异性
- Cell surface
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纯化方法
- Stimulated human leukocytes were used as the immunogen for the CD43 antibody.
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免疫原
- Stimulated human leukocytes were used as the immunogen for the CD43 antibody.
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应用备注
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- CD43 (SPN) (Sialophorin (SPN))
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别名
- Leukosialin
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背景
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Target Description: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Gene Symbol: SPN///CD43
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基因ID
- 6693
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UniProt
- P16150
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途径
- Regulation of Leukocyte Mediated Immunity
抗原
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