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Keratin 77 抗体

This 小鼠 单克隆 antibody specifically detects Keratin 77 in IHC, WB, IF 和 FACS. It exhibits reactivity toward 人 和 大鼠.
产品编号 ABIN6297232
发货至: 中国
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Quick Overview for Keratin 77 抗体 (ABIN6297232)

抗原

See all Keratin 77 (KRT77) 抗体
Keratin 77 (KRT77)

适用

  • 19
  • 6
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
人, 大鼠

宿主

  • 12
  • 7
小鼠

克隆类型

  • 10
  • 9
单克隆

标记

  • 15
  • 2
  • 1
  • 1
This Keratin 77 antibody is un-conjugated

应用范围

  • 10
  • 9
  • 6
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  • 3
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  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 原理

    Mouse anti-Human/Rat Acidic Cytokeratin Antibody [Sodium Azide Free]

    特异性

    Cytoplasmic

    纯化方法

    Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.

    免疫原

    Recombinant human KRT77 protein was used as the immunogen for the Acidic Cytokeratin antibody.
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL
    Western blot: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    Keratin 77 (KRT77)

    别名

    Keratin, type II cytoskeletal 1b

    背景

    Target Description: This mAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

    Gene Symbol: KRT77///KRT1B

    基因ID

    374454

    UniProt

    Q7Z794
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