Nuclear Marker 抗体
Quick Overview for Nuclear Marker 抗体 (ABIN6296760)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human/Mouse/Rat Nuclear Marker Antibody [Sodium Azide Free]
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特异性
- Nuclei
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纯化方法
- Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
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免疫原
- Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
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anti-Nuclear Marker antibody
适用: 人, 小鼠, 大鼠 FACS, IF, ICC, IHC (p) 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Marker antibody (PE)适用: 人, 灵长类 FACS, IF 宿主: 小鼠 Monoclonal 235-1 PE
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Nuclear Marker
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背景
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Target Description: This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
抗原
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