Keratin 77 抗体
Quick Overview for Keratin 77 抗体 (ABIN6296409)
抗原
See all Keratin 77 (KRT77) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken pan Cytokeratin Antibody Cocktail [Sodium Azide Free]
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特异性
- Cytoplasmic
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纯化方法
- Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.
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免疫原
- Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Keratin 77 (KRT77)
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别名
- Keratin, type II cytoskeletal 1b
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背景
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Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
Gene Symbol: KRT77///KRT1B///KRT76///KRT2B///KRT2P
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UniProt
- Q7Z794, Q01546
抗原
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