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Keratin 77 抗体

This anti-Keratin 77 antibody is a 小鼠 单克隆 antibody detecting Keratin 77 in IHC, WB, IF 和 FACS. Suitable for 人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡 和 猴.
产品编号 ABIN6296409
发货至: 中国

Quick Overview for Keratin 77 抗体 (ABIN6296409)

抗原

See all Keratin 77 (KRT77) 抗体
Keratin 77 (KRT77)

适用

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人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This Keratin 77 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 原理

    Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken pan Cytokeratin Antibody Cocktail [Sodium Azide Free]

    特异性

    Cytoplasmic

    纯化方法

    Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

    免疫原

    Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL
    Western blot: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    Keratin 77 (KRT77)

    别名

    Keratin, type II cytoskeletal 1b

    背景

    Target Description: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

    Gene Symbol: KRT77///KRT1B///KRT76///KRT2B///KRT2P

    UniProt

    Q7Z794, Q01546
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