KRT8 抗体
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北京 101111
Quick Overview for KRT8 抗体 (ABIN6295957)
抗原
See all KRT8 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human/Monkey/Cow/Sheep/Pig Cytokeratin 8 + 18 Antibody [Sodium Azide Free]
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特异性
- Cytoplasmic
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纯化方法
- A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8 + 18 antibody.
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免疫原
- A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8 + 18 antibody.
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- KRT8 (Keratin 8 (KRT8))
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别名
- Keratin, type II cytoskeletal 8
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背景
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Target Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Gene Symbol: KRT8///CYK8///KRT18///CYK18///PIG46
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UniProt
- P05787, P05783
抗原
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