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KRT8 抗体

This 小鼠 单克隆 antibody specifically detects KRT8 in WB, IHC, IF 和 FACS. It exhibits reactivity toward 人, 大鼠 和 斑马鱼.
产品编号 ABIN6295954
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for KRT8 抗体 (ABIN6295954)

抗原

See all KRT8 抗体
KRT8 (Keratin 8 (KRT8))

适用

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人, 大鼠, 斑马鱼

宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT8 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 原理

    Mouse anti-Human/Rat/Zebrafish Cytokeratin 8 Antibody [Sodium Azide Free]

    特异性

    Cytoplasmic

    纯化方法

    A cytoskeleton preparation containing Cytokeratin 8 was used as the immunogen for this antibody.

    免疫原

    A cytoskeleton preparation containing Cytokeratin 8 was used as the immunogen for this antibody.
  • 应用备注

    Western blot: 1-2 μg/mL
    FACS: 0.5-1 μg/million cells
    IF: 1-2 μg/mL
    IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS ( pH 7.4), BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    KRT8 (Keratin 8 (KRT8))

    别名

    Keratin, type II cytoskeletal 8

    背景

    Target Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. antibody to Cytokeratin 8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against Cytokeratin 8, 18 and 19). Reportedly, Cytokeratin 8 antibody is useful for the differentiation of lobular ( ""ring-like, perinuclear "") from ductal ( ""peripheral-predominant "") carcinoma of the breast.

    Gene Symbol: KRT8///CYK8

    基因ID

    3856

    UniProt

    P05787
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