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EpCAM 抗体 (N-Term)

The 小鼠 单克隆 anti-EpCAM antibody (ABIN6295704) specifically detects EpCAM in WB, FACS, IHC 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN6295704
发货至: 中国
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Quick Overview for EpCAM 抗体 (N-Term) (ABIN6295704)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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小鼠

克隆类型

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单克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
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    AA 20-60, N-Term

    原理

    Mouse anti-Human EpCAM Antibody [Sodium Azide Free]

    特异性

    Cell surface

    纯化方法

    A synthetic peptide (around aa 20-60) from the N-terminus of the human protein was used as the immunogen for the EpCAM antibody.

    免疫原

    A synthetic peptide (around aa 20-60) from the N-terminus of the human protein was used as the immunogen for the EpCAM antibody.
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL
    Western blot: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    Epithelial cell adhesion molecule

    背景

    Target Description: Recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

    Gene Symbol: EPCAM///GA733-2///M1S2///M4S1///MIC18///TACSTD1///TROP1

    基因ID

    4072

    UniProt

    P16422
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