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KRT8 抗体 (AA 343-357)

The 小鼠 单克隆 anti-KRT8 antibody is suitable to detect KRT8 in samples from 人. It has been validated for IHC, IF 和 FACS.
产品编号 ABIN6294485
发货至: 中国
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Quick Overview for KRT8 抗体 (AA 343-357) (ABIN6294485)

抗原

See all KRT8 抗体
KRT8 (Keratin 8 (KRT8))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT8 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
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    AA 343-357

    原理

    Mouse anti-Human Cytokeratin 8 Antibody [Sodium Azide Free]

    特异性

    Cytoplasmic

    纯化方法

    A keratin preparation from a human carcinoma was used as the immunogen for this Cytokeratin 8 antibody. The epitope of this Ab is located between amino acids 343-357.

    免疫原

    A keratin preparation from a human carcinoma was used as the immunogen for this Cytokeratin 8 antibody. The epitope of this Ab is located between amino acids 343-357.
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    KRT8 (Keratin 8 (KRT8))

    别名

    Keratin, type II cytoskeletal 8

    背景

    Target Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.

    Gene Symbol: KRT8///CYK8

    基因ID

    3856

    UniProt

    P05787
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