Myogenin 抗体 (AA 30-224)
Quick Overview for Myogenin 抗体 (AA 30-224) (ABIN6294456)
抗原
See all Myogenin (MYOG) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 30-224
-
原理
- Mouse anti-Human/Mouse/Rat/Cat/Pig Myogenin Antibody Cocktail [Sodium Azide Free]
-
特异性
- Nuclear
-
纯化方法
- Full length human recombinant protein (MGN185) and a partial rat recombinant protein (amino acid 30-224) (F5D) were used as the immunogens for this Myogenin antibody cocktail.
-
免疫原
- Full length human recombinant protein (MGN185) and a partial rat recombinant protein (amino acid 30-224) (F5D) were used as the immunogens for this Myogenin antibody cocktail.
-
-
-
-
应用备注
-
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
缓冲液
- In 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
-
-
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
-
别名
- Myogenin
-
背景
-
Target Description: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
Gene Symbol: MYOG///BHLHC3///MYF4
-
基因ID
- 4656
-
UniProt
- P15173
-
途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
-