Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2) (AA 280-420) 抗体
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Quick Overview for Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2) (AA 280-420) 抗体 (ABIN6294441)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 280-420
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原理
- Mouse anti-Human IDH1 Antibody [IHC only]
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特异性
- Cytoplasmic & nuclear
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纯化方法
- A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.
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免疫原
- A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.
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应用备注
- Prediluted format : incubate for 30 min at RT (2)
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限制
- 仅限研究用
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缓冲液
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, For IHC use only
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2)
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别名
- Isocitrate dehydrogenase cytoplasmic
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背景
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Target Description: It recognizes a 45 kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming a-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of a-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
Gene Symbol: IDH1///PICD
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基因ID
- 3417
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UniProt
- O75874
抗原
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