Combined Host Cell Proteins 抗体 (Biotin)
适用: E. coli
ELISA, WB
宿主: 兔
Polyclonal
Biotin
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- 抗原
- Combined Host Cell Proteins
- 适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
- 应用范围
- ELISA, Western Blotting (WB)
- 原理
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
- 特异性
- Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.
- 交叉反应 （详细）
- Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
- 产品特性
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
- 纯化方法
- The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins.
- 免疫原
- Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
- 亚型
- IgG
- Labeling Ratio
- 10-20 moles Biotin per mole of IgG
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- 应用备注
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ELISA : 1:1000-1:2000
Western Blot : 1:500-1:1000 - 说明
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Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with 100 μL deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Combined Host Cell Proteins
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