Combined Host Cell Proteins 抗体 (Biotin)
Quick Overview for Combined Host Cell Proteins 抗体 (Biotin) (ABIN6266438)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-406-m61
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Supplier
- Rockland
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原理
- Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
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产品特性
- Synonyms: rabbit anti-Host Cell Protein antibody Biotin conjugation, rabbit anti-HCP antibody, rabbit anti-E.Coli HCP antibody biotin conjugated, Anti-HCP biotin
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纯化方法
- Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.
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免疫原
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Immunogen: Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
Immunogen Type: Native Protein
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亚型
- IgG
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Labeling Ratio
- 10-20
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anti-Combined Host Cell Proteins antibody
适用: E. coli ELISA, WB, 2D-PAGE 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by western blot and is suitable for ELISA.
Western Blot Dilution: 1:500-1:1000
ELISA Dilution: 1:1000-1:2000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Combined Host Cell Proteins
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背景
- Background: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
抗原
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