PIWIL4 抗体 (N-Term)
Quick Overview for PIWIL4 抗体 (N-Term) (ABIN6266377)
抗原
See all PIWIL4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- PIWIL4 Antibody detects endogenous levels of total PIWIL4.
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预测反应
- Horse
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human PIWIL4, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Piwi-Like 4 (PIWIL4) (AA 304-434) antibody
PIWIL4 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 10G9B11 unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 260-460) antibodyPIWIL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) antibodyPIWIL4 适用: 人, 小鼠, 大鼠 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 12-235) antibodyPIWIL4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 1-852) antibodyPIWIL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) antibodyPIWIL4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) antibodyPIWIL4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 1-852) antibodyPIWIL4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 35-84) antibodyPIWIL4 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- PIWIL4 (Piwi-Like 4 (PIWIL4))
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别名
- PIWIL4
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背景
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Description: Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity). Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity). The piRNA process acts upstream of known mediators of DNA methylation (By similarity). Does not show endonuclease activity (By similarity). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373).
Gene: PIWIL4
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分子量
- 97kDa
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基因ID
- 143689
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UniProt
- Q7Z3Z4
抗原
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