PIWIL2 抗体 (C-Term)
Quick Overview for PIWIL2 抗体 (C-Term) (ABIN6266308)
抗原
See all PIWIL2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- PIWIL2 Antibody detects endogenous levels of total PIWIL2.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human PIWIL2, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Piwi-Like 2 (PIWIL2) (AA 30-80) antibody
PIWIL2 适用: 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 2 (PIWIL2) antibodyPIWIL2 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 2 (PIWIL2) (AA 1-973) antibodyPIWIL2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Piwi-Like 2 (PIWIL2) (AA 287-336) antibodyPIWIL2 适用: 人, Cow, 犬, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 2 (PIWIL2) (AA 90-119), (N-Term) antibodyPIWIL2 适用: 人 WB 宿主: 兔 Polyclonal RB39798 unconjugated
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应用备注
- WB 1:500-1:1000, IHC 1:20-1:50, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- PIWIL2 (Piwi-Like 2 (PIWIL2))
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别名
- PIWIL2
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背景
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Description: Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (By similarity). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (By similarity). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (By similarity). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (By similarity). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660).
Gene: PIWIL2
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分子量
- 110kDa
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基因ID
- 55124
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UniProt
- Q8TC59
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途径
- Stem Cell Maintenance
抗原
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