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YTHDF1 抗体 (N-Term)

This 兔 多克隆 anti-YTHDF1 antibody specifically detects YTHDF1 in WB, ELISA, IF 和 ICC. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN6266080
发货至: 中国
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Quick Overview for YTHDF1 抗体 (N-Term) (ABIN6266080)

抗原

YTHDF1 (YTH Domain Family, Member 1 (YTHDF1))

适用

  • 23
  • 12
  • 9
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 26

克隆类型

  • 21
  • 5
多克隆

标记

  • 19
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This YTHDF1 antibody is un-conjugated

应用范围

  • 17
  • 9
  • 6
  • 5
  • 2
  • 2
  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    特异性

    YTHDF1 Antibody detects endogenous levels of total YTHDF1.

    预测反应

    Pig,Bovine,Horse,Sheep,Chicken,Xenopus

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human YTHDF1, corresponding to a region within N-terminal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    YTHDF1 (YTH Domain Family, Member 1 (YTHDF1))

    别名

    YTHDF1

    背景

    Description: Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440).

    Gene: YTHDF1

    分子量

    61kDa

    基因ID

    54915

    UniProt

    Q9BYJ9
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