USP7 抗体 (Internal Region)
Quick Overview for USP7 抗体 (Internal Region) (ABIN6265935)
抗原
See all USP7 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
特异性
- USP7 Antibody detects endogenous levels of total USP7.
-
预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human USP7, corresponding to a region within the internal amino acids.
-
亚型
- IgG
-
-
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 54-507) antibody
USP7 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) antibodyUSP7 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 7F10 unconjugated Recombinant Antibody
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 206-1102) antibodyUSP7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 1050-1100) antibodyUSP7 适用: 人, 小鼠, 大鼠, 犬, 马, 小鸡, Macaque WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 258-483) antibodyUSP7 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 302-394), (C-Term) antibodyUSP7 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 30-80) antibodyUSP7 适用: 人, 小鼠, 大鼠, 犬, 马, 小鸡, Macaque WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) antibodyUSP7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5F11 unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) (AA 1060-1090), (C-Term) antibodyUSP7 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4281 unconjugated
anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) antibodyUSP7 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:20-1:50, ELISA(peptide) 1:20000-1:40000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
-
有效期
- 12 months
-
-
- USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))
-
别名
- USP7
-
背景
-
Description: Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148, PubMed:26678539). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation (PubMed:15053880, PubMed:16845383, PubMed:18566590, PubMed:20153724). Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis (PubMed:11923872). Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity (PubMed:16964248). In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML (PubMed:18716620). Deubiquitinates KMT2E/MLL5 preventing KMT2E/MLL5 proteasomal-mediated degradation (PubMed:26678539). Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6 (PubMed:22466611, PubMed:22466612). Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816, PubMed:22411829). Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex, may act by deubiquitinating components of the PRC1-like complex (PubMed:20601937). Able to mediate deubiquitination of histone H2B, it is however unsure whether this activity takes place in vivo (PubMed:20601937). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains (PubMed:22689415). Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).
Gene: USP7
-
分子量
- 130kDa
-
基因ID
- 7874
-
UniProt
- Q93009
抗原
-
