SH2B1 抗体 (Internal Region)
Quick Overview for SH2B1 抗体 (Internal Region) (ABIN6265047)
抗原
See all SH2B1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- SH2B1 Antibody detects endogenous levels of total SH2B1.
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预测反应
- Horse,Sheep,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human SH2B1, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-SH2B Adaptor Protein 1 (SH2B1) antibody
SH2B1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 327-426) antibodySH2B1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2B9 unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 1-426) antibodySH2B1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 360-520) antibodySH2B1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 395-444) antibodySH2B1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 1-426) antibodySH2B1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) antibodySH2B1 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- SH2B1 (SH2B Adaptor Protein 1 (SH2B1))
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别名
- SH2B1
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背景
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Description: Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway, prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis (By similarity).
Gene: SH2B1
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分子量
- 79kDa
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基因ID
- 25970
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UniProt
- Q9NRF2
抗原
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