PUM2 抗体 (N-Term)
Quick Overview for PUM2 抗体 (N-Term) (ABIN6264530)
抗原
See all PUM2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- PUM2 Antibody detects endogenous levels of total PUM2.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human PUM2, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Pumilio Homolog 2 (Drosophila) (PUM2) (Internal Region) antibody
Verified PUM2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 106-205) antibodyPUM2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3E6 unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) antibodyPUM2 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 159-189) antibodyPUM2 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB15584 unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (Ser182) antibodyPUM2 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 701-798) antibodyPUM2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 106-205) antibodyPUM2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F10 unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 701-798) antibodyPUM2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7C7 unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 106-205) antibodyPUM2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E10 unconjugated
anti-Pumilio Homolog 2 (Drosophila) (PUM2) (AA 101-150) antibodyPUM2 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, Pig, Bat, 小鸡, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))
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别名
- PUM2
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背景
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Description: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). May regulate DCUN1D3 mRNA levels (PubMed:25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233).
Gene: PUM2
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分子量
- 114 kDa
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基因ID
- 23369
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UniProt
- Q8TB72
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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