POLR2D 抗体 (N-Term)
Quick Overview for POLR2D 抗体 (N-Term) (ABIN6264344)
抗原
See all POLR2D 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- POLR2D Antibody detects endogenous levels of total POLR2D.
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预测反应
- Pig,Bovine,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human POLR2D, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- POLR2D (Polymerase (RNA) II (DNA Directed) Polypeptide D (POLR2D))
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别名
- POLR2D
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背景
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Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).
Gene: POLR2D
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分子量
- 16kDa
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基因ID
- 5433
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UniProt
- O15514
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途径
- Regulatory RNA Pathways
抗原
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