PDPK1 抗体 (Internal Region)
Quick Overview for PDPK1 抗体 (Internal Region) (ABIN6264115)
抗原
See all PDPK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- PDPK1 Antibody detects endogenous levels of total PDPK1.
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预测反应
- Pig,Bovine,Horse,Sheep,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human PDPK1, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 150-429) antibodyPDPK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) antibodyPDPK1 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-150) antibodyPDPK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibodyPDPK1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7E4G11 unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibodyPDPK1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4A11 unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibodyPDPK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 83-338) antibodyPDPK1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibodyPDPK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2 unconjugated
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pTyr373), (pTyr376) antibodyPDPK1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
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别名
- PDPK1
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背景
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Description: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.
Gene: PDPK1
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分子量
- 63kDa
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基因ID
- 5170
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UniProt
- O15530
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途径
- PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
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