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NDC80 抗体 (C-Term)

This anti-NDC80 antibody is a 兔 多克隆 antibody detecting NDC80 in WB, ELISA, IHC, IF 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN6263548
发货至: 中国

Quick Overview for NDC80 抗体 (C-Term) (ABIN6263548)

抗原

See all NDC80 抗体
NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This NDC80 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

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    C-Term

    特异性

    NDC80 Antibody detects endogenous levels of total NDC80.

    预测反应

    Dog

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human NDC80, corresponding to a region within C-terminal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

    别名

    NDC80

    背景

    Description: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020). Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules (PubMed:25743205, PubMed:23891108).

    Gene: NDC80

    分子量

    74kDa

    基因ID

    10403

    UniProt

    O14777

    途径

    Maintenance of Protein Location
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