LATS1,2 (Internal Region) 抗体
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北京 101111
Quick Overview for LATS1,2 (Internal Region) 抗体 (ABIN6262920)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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原理
- LATS1/2 Antibody
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特异性
- LATS1/2 Antibody detects endogenous levels of total LATS1/2.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human LATS1/2, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-LATS1,2 (AA 641-740) antibody
适用: 人 WB, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-LATS1,2 (Ser116) antibody适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:1000-3000, IHC 1:50-1:200 IF/ICC 1:100-1:500
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- LATS1,2
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别名
- LATS1/2
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背景
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Description: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668).
Gene: LATS1
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分子量
- 160 kDa
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基因ID
- 9113
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UniProt
- O95835
抗原
-
