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Interleukin enhancer-binding factor 3 (ILF3) (Internal Region) 抗体

This anti-Interleukin enhancer-binding factor 3 antibody is a 兔 多克隆 antibody detecting Interleukin enhancer-binding factor 3 in WB, IHC, ELISA, IF 和 ICC. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN6262571
发货至: 中国
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Quick Overview for Interleukin enhancer-binding factor 3 (ILF3) (Internal Region) 抗体 (ABIN6262571)

抗原

See all Interleukin enhancer-binding factor 3 (ILF3) 抗体
Interleukin enhancer-binding factor 3 (ILF3)

适用

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人, 小鼠, 大鼠

宿主

  • 66
  • 2
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克隆类型

  • 48
  • 22
多克隆

标记

  • 47
  • 3
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非结合性

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

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    Internal Region

    特异性

    ILF3 Antibody detects endogenous levels of total ILF3.

    预测反应

    Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human ILF3, corresponding to a region within the internal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    Interleukin enhancer-binding factor 3 (ILF3)

    别名

    ILF3

    背景

    Description: RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 Molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).

    Gene: ILF3

    分子量

    95kDa

    基因ID

    3609

    UniProt

    Q12906

    途径

    M Phase
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