GSDMD 抗体
Quick Overview for GSDMD 抗体 (ABIN6262106)
抗原
See all GSDMD 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- GSDMD Antibody detects endogenous levels of total GSDMD.
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预测反应
- Pig,Bovine,Horse,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human GSDMD.
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亚型
- IgG
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anti-Gasdermin D (GSDMD) (AA 1-484) antibody
GSDMD 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F12-1B2 unconjugated
anti-Gasdermin D (GSDMD) (AA 276-484) antibodyGSDMD 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-485) antibodyGSDMD 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F12-1B2 unconjugated
anti-Gasdermin D (GSDMD) antibodyGSDMD 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-326) antibodyGSDMD 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-256) antibodyGSDMD 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 232-484) antibodyGSDMD 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-484) antibodyGSDMD 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-484) antibodyGSDMD 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200 IF/ICC 1:200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- GSDMD (Gasdermin D (GSDMD))
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别名
- GSDMD
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背景
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Description: Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
Gene: GSDMD
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分子量
- 50-53 kDa
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基因ID
- 79792
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UniProt
- P57764
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途径
- Inflammasome
抗原
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