SR Proteins 抗体
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北京 101111
Quick Overview for SR Proteins 抗体 (ABIN626151)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Detects a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species cross-reactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.
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纯化方法
- Protein A purified
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免疫原
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Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST.
Type of Immunogen: GST fusion protein -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IF (0.5 - 2 μg/mL), IHC, IHC-P (10 μg/mL), WB (3 - 5 μg/mL)
Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot. Reactivity is confirmed by Western Blot. ELISA: 0.1-1 μg/mL. Immunofluorescence: 0.5-2 μg/mL, human. Western Blot: 3-5 μg/mL. Positive control: HeLa cell, nuclear extracts. -
说明
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Target Species of Antibody: Bovine
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Aliquot to Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
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- SR Proteins
抗原
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