CENPE 抗体 (C-Term)
Quick Overview for CENPE 抗体 (C-Term) (ABIN6260750)
抗原
See all CENPE 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- CENPE Antibody detects endogenous levels of total CENPE.
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预测反应
- Pig,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human CENPE, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Centromere Protein E, 312kDa (CENPE) antibody
CENPE 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人 IP, ICC, IF, FACS 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2450-2590) antibodyCENPE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (C-Term) antibodyCENPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人 WB, IP, ICC, IF 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2542-2701) antibodyCENPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2222-2361) antibodyCENPE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- CENPE (Centromere Protein E, 312kDa (CENPE))
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别名
- CENPE
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背景
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Description: Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed:7889940, PubMed:23891108, PubMed:25395579). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed:25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips, after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed:23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed:25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).
Gene: CENPE
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分子量
- 312 kDa
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基因ID
- 1062
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UniProt
- Q02224
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途径
- M Phase, Maintenance of Protein Location
抗原
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