Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (N-Term) 抗体
Quick Overview for Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (N-Term) 抗体 (ABIN6260515)
抗原
See all Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- CBLB Antibody detects endogenous levels of total CBLB.
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预测反应
- Pig,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human CBLB, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 101-200) antibody
CBLB 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 1-350) antibodyCBLB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 103-130) antibodyCBLB 适用: 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21574 unconjugated
anti-Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) antibodyCBLB 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (C-Term) antibodyCBLB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) antibodyCBLB 适用: 人 WB, DB 宿主: 小鼠 Monoclonal 246C5a unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)
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别名
- CBLB
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背景
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Description: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Gene: CBLB
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分子量
- 109kDa
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基因ID
- 868
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UniProt
- Q13191
抗原
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