BRD4 抗体 (N-Term)
Quick Overview for BRD4 抗体 (N-Term) (ABIN6260309)
抗原
See all BRD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- Brd4 Antibody detects endogenous levels of total Brd4.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human Brd4, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Bromodomain Containing 4 (BRD4) antibody
BRD4 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) antibodyBRD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 1E5 unconjugated Recombinant Antibody
anti-Bromodomain Containing 4 (BRD4) (AA 1313-1342), (C-Term) antibodyBRD4 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB3422 unconjugated
anti-Bromodomain Containing 4 (BRD4) (C-Term) antibodyBRD4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马 WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 150-200) antibodyBRD4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 1-30), (N-Term) antibodyBRD4 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB3419 unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 651-700) antibodyBRD4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 700-794) antibodyBRD4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) antibodyBRD4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (pThr204) antibodyBRD4 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
- WB 1:1000-3000, IHC 1:200, IF/ICC, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- BRD4 (Bromodomain Containing 4 (BRD4))
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别名
- BRD4
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背景
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Description: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.
Gene: BRD4
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分子量
- 152kDa
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基因ID
- 23476
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UniProt
- O60885
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途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome
抗原
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