ATRX 抗体 (N-Term)
Quick Overview for ATRX 抗体 (N-Term) (ABIN6260147)
抗原
See all ATRX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- ATRX Antibody detects endogenous levels of total ATRX.
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预测反应
- Bovine,Rabbit,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human ATRX, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-helicase 2, X-linked (ATRX) (C-Term) antibody
ATRX 适用: 人, 小鼠 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-helicase 2, X-linked (ATRX) (AA 2161-2443) antibodyATRX 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-helicase 2, X-linked (ATRX) (AA 2311-2492) antibodyATRX 适用: 人 ELISA 宿主: 小鼠 Monoclonal 8B2H9 unconjugated
anti-helicase 2, X-linked (ATRX) (AA 2311-2410) antibodyATRX 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 5B3 unconjugated
anti-helicase 2, X-linked (ATRX) antibodyATRX 适用: 人 IHC, ISt, StM 宿主: 兔 Monoclonal ATRX-2900R unconjugated Recombinant Antibody
anti-helicase 2, X-linked (ATRX) antibodyATRX 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal 39f unconjugated
anti-helicase 2, X-linked (ATRX) (N-Term) antibodyATRX 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-helicase 2, X-linked (ATRX) antibodyATRX 适用: 人 ELISA, Coat 宿主: 小鼠 Monoclonal 23c unconjugated
anti-helicase 2, X-linked (ATRX) (C-Term) antibodyATRX 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-helicase 2, X-linked (ATRX) (AA 634-663), (C-Term) antibodyATRX 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB20635 unconjugated
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应用备注
- IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- ATRX (helicase 2, X-linked (ATRX))
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别名
- ATRX
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背景
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Description: Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting, according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).
Gene: ATRX
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分子量
- 282kDa
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基因ID
- 546
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UniProt
- P46100
抗原
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