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Pre-mRNA Branch Site Protein p14 (SF3B14) (C-Term) 抗体

The 兔 多克隆 anti-Pre-mRNA Branch Site Protein p14 antibody is suitable to detect Pre-mRNA Branch Site Protein p14 in samples from 人, 小鼠 和 大鼠. It has been validated for WB, ELISA, IHC, IF 和 ICC.
产品编号 ABIN6259614
发货至: 中国
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Quick Overview for Pre-mRNA Branch Site Protein p14 (SF3B14) (C-Term) 抗体 (ABIN6259614)

抗原

See all Pre-mRNA Branch Site Protein p14 (SF3B14) 抗体
Pre-mRNA Branch Site Protein p14 (SF3B14)

适用

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人, 小鼠, 大鼠

宿主

  • 29

克隆类型

  • 29
多克隆

标记

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非结合性

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

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    C-Term

    特异性

    SF3B14 Antibody detects endogenous levels of total SF3B14.

    预测反应

    Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human SF3B14, corresponding to a region within C-terminal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    Pre-mRNA Branch Site Protein p14 (SF3B14)

    别名

    SF3B6

    背景

    Description: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA (PubMed:12234937). Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively (PubMed:16432215).

    Gene: SF3B6

    分子量

    20 kDa

    基因ID

    51639

    UniProt

    Q9Y3B4
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