Pre-mRNA Branch Site Protein p14 (SF3B14) (C-Term) 抗体
Quick Overview for Pre-mRNA Branch Site Protein p14 (SF3B14) (C-Term) 抗体 (ABIN6259614)
抗原
See all Pre-mRNA Branch Site Protein p14 (SF3B14) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- SF3B14 Antibody detects endogenous levels of total SF3B14.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human SF3B14, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- Pre-mRNA Branch Site Protein p14 (SF3B14)
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别名
- SF3B6
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背景
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Description: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA (PubMed:12234937). Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively (PubMed:16432215).
Gene: SF3B6
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分子量
- 20 kDa
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基因ID
- 51639
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UniProt
- Q9Y3B4
抗原
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