TOP3B 抗体 (C-Term)
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北京 101111
Quick Overview for TOP3B 抗体 (C-Term) (ABIN6259219)
抗原
See all TOP3B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- TOP3B Antibody detects endogenous levels of total TOP3B.
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预测反应
- Rabbit
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human TOP3B, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibody
TOP3B 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4F11 unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibodyTOP3B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-250) antibodyTOP3B 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (N-Term) antibodyTOP3B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) antibodyTOP3B 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) III beta (TOP3B) (AA 1-862) antibodyTOP3B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- TOP3B (Topoisomerase (DNA) III beta (TOP3B))
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别名
- TOP3B
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背景
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Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Gene: TOP3B
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分子量
- 96 kDa
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基因ID
- 8940
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UniProt
- O95985
抗原
-
