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- 抗原 See all SRPK1 抗体
- SRPK1 (SRSF Protein Kinase 1 (SRPK1))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SRPK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- SRPK1 Antibody detects endogenous levels of total SRPK1.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human SRPK1, corresponding to a region within C-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product SRPK1 Primary Antibody
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anti-SRSF Protein Kinase 1 (SRPK1) (AA 371-470) antibody
SRPK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 6H5 unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) antibodySRPK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 290-480) antibodySRPK1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 371-470) antibodySRPK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 1-655) antibodySRPK1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 371-470) antibodySRPK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G12 unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 1-30), (N-Term) antibodySRPK1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB3355 unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 538-567), (C-Term) antibodySRPK1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB9188 unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) antibodySRPK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-SRSF Protein Kinase 1 (SRPK1) (AA 399-448) antibodySRPK1 适用: 人, 犬, 豚鼠, 兔, Pig, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- SRPK1 (SRSF Protein Kinase 1 (SRPK1))
- 别名
- SRPK1 (SRPK1 产品)
- 别名
- SFRSK1 antibody, AU017960 antibody, BEST:CK01209 antibody, CG11489 antibody, CG9085 antibody, CK01209 antibody, Dmel\\CG11489 antibody, SRPK antibody, SRPK1 antibody, Srpk79D antibody, atc antibody, srpk1 antibody, zgc:63789 antibody, wu:fc06h03 antibody, zgc:158800 antibody, sfrsk1 antibody, SRSF protein kinase 1 antibody, serine/arginine-rich protein specific kinase 1 antibody, Serine-arginine protein kinase at 79D antibody, SRSF protein kinase 1a antibody, SRSF protein kinase 1b antibody, SRSF protein kinase 1 S homeolog antibody, SRPK1 antibody, Srpk1 antibody, Srpk79D antibody, srpk1a antibody, srpk1b antibody, srpk1.S antibody
- 背景
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Description: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.
Gene: SRPK1
- 分子量
- 95 kDa
- 基因ID
- 6732
- UniProt
- Q96SB4
- 途径
- Toll-Like Receptors Cascades
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