PRP19 抗体 (N-Term)
Quick Overview for PRP19 抗体 (N-Term) (ABIN6259137)
抗原
See all PRP19 (PRPF19) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- PRPF19 Antibody detects endogenous levels of total PRPF19.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human PRPF19, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Pre-mRNA Processing Factor 19 (PRPF19) (AA 1-90) antibody
PRPF19 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E5 unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) (AA 127-416) antibodyPRPF19 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) (AA 1-90) antibodyPRPF19 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) antibodyPRPF19 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) (AA 4-33), (N-Term) antibodyPRPF19 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20845 unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) (Middle Region) antibodyPRPF19 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 斑马鱼, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) (AA 151-200) antibodyPRPF19 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 斑马鱼, 豚鼠, Pig, Bat, 猴, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) antibodyPRPF19 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) (Internal Region) antibodyPRPF19 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 19 (PRPF19) antibodyPRPF19 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))
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别名
- PRPF19
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背景
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Description: Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).
Gene: PRPF19
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分子量
- 50 kDa
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基因ID
- 27339
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UniProt
- Q9UMS4
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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