MHC Class II RT-1B 抗体
This anti-MHC Class II RT-1B antibody (ABIN625901) is a Mouse Monoclonal antibody detecting MHC Class II RT-1B in FACS, IHC (fro), IHC (p), WB, IHC. Suitable for Rat, Mouse.
Quick Overview for MHC Class II RT-1B 抗体 (ABIN625901)
抗原
MHC Class II RT-1B
适用
大鼠, 小鼠
宿主
小鼠
克隆类型
单克隆
标记
This MHC Class II RT-1B antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)
克隆位点
OX-6
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品牌
- IHC-plus™
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特异性
- Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.
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纯化方法
- Protein G purified
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免疫原
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Rat thymocyte membrane glycoprotein.
Type of Immunogen: Membrane extract -
亚型
- IgG1
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anti-MHC Class II RT-1B antibody
适用: 大鼠, 小鼠 FACS, IHC (fro), IHC (p), WB, IF 宿主: 小鼠 Monoclonal OX-6 unconjugated
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应用备注
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Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MHC Class II RT-1B
抗原
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