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Doublecortin 抗体 (AA 232-242)

This anti-Doublecortin antibody is a 山羊 多克隆 antibody detecting Doublecortin in WB, ELISA, IHC 和 IF. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN625829
发货至: 中国

Quick Overview for Doublecortin 抗体 (AA 232-242) (ABIN625829)

抗原

See all Doublecortin (DCX) 抗体
Doublecortin (DCX)

适用

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人, 小鼠, 大鼠

宿主

  • 95
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山羊

克隆类型

  • 91
  • 22
多克隆

标记

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This Doublecortin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    AA 232-242

    原理

    Doublecortin / DCX (aa232-242)

    序列

    KTSANMKAPQ S

    特异性

    This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    CKTSANMKAPQS

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4 μg/mL. This antibody has been successfully used in IHC on Rat: Heysieattalab, S et al. (2021) Acta Epileptologica 3, 3

    Western Blot: Approx 45 kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3 kDa according to Mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells. Recommended concentration: 5-10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Doublecortin (DCX)

    别名

    DCX

    背景

    DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin

    基因ID

    1641, 13193, 84394

    NCBI登录号

    NP_000546, NP_835365, NP_835364, NP_001182482
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