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SDF4 抗体 (AA 161-175)

This anti-SDF4 antibody is a 山羊 多克隆 antibody detecting SDF4 in WB, ELISA, IHC, FACS 和 IF. Suitable for 人.
产品编号 ABIN625824
发货至: 中国

Quick Overview for SDF4 抗体 (AA 161-175) (ABIN625824)

抗原

See all SDF4 抗体
SDF4 (Stromal Cell Derived Factor 4 (SDF4))

适用

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宿主

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山羊

克隆类型

  • 39
多克隆

标记

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This SDF4 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    AA 161-175

    原理

    SDF4 (aa161-175)

    序列

    EYKVKFLASK GHSEK

    特异性

    This antibody is expected to recognize both reported isoforms NP_057631.2, and NP_057260.3

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    CEYKVKFLASKGHSEK

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10 μg/mL.

    Western Blot: Approx 45 kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8 kDa according to NP_057260.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    SDF4 (Stromal Cell Derived Factor 4 (SDF4))

    别名

    SDF4

    背景

    SDF4, stromal cell derived factor 4, Cab45, 45 kDa calcium-binding protein, OTTHUMP00000001754, SDF-4, calcium binding protein, stromal cell-derived factor 4

    基因ID

    51150, 20318, 155173

    NCBI登录号

    NP_057631, NP_057260
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