EIF5B 抗体 (C-Term)
Quick Overview for EIF5B 抗体 (C-Term) (ABIN6257600)
抗原
See all EIF5B 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
特异性
- EIF5B Antibody detects endogenous levels of total EIF5B.
-
预测反应
- Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human EIF5B, corresponding to a region within C-terminal amino acids.
-
亚型
- IgG
-
-
-
-
应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
-
有效期
- 12 months
-
-
- EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))
-
别名
- EIF5B
-
背景
-
Description: Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
Gene: EIF5B
-
分子量
- 139 kDa
-
基因ID
- 9669
-
UniProt
- O60841
抗原
-