NDFIP1 抗体 (C-Term)
Quick Overview for NDFIP1 抗体 (C-Term) (ABIN6257368)
抗原
See all NDFIP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- NDFIP1 Antibody detects endogenous levels of total NDFIP1.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human NDFIP1, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Nedd4 Family Interacting Protein 1 (NDFIP1) (AA 2-116) antibody
NDFIP1 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nedd4 Family Interacting Protein 1 (NDFIP1) (C-Term) antibodyNDFIP1 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Nedd4 Family Interacting Protein 1 (NDFIP1) (AA 1-221) antibodyNDFIP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Nedd4 Family Interacting Protein 1 (NDFIP1) (AA 1-116) antibodyNDFIP1 适用: 人 EIA, RIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- NDFIP1 (Nedd4 Family Interacting Protein 1 (NDFIP1))
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别名
- NDFIP1
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背景
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Description: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cell-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). Promotes pancreatic beta cell death through degradation of JUNB and inhibition of the unfolded protein response, leading to reduction of insulin secretion (PubMed:26319551). Restricts the production of proinflammatory cytokines in effector Th17 T-cells by promoting ITCH-mediated ubiquitination and degradation of RORC (By similarity). Together with NDFIP2, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity). Regulates peripheral T-cell tolerance to self and foreign antigens, forcing the exit of naive CD4+ T-cells from the cell cycle before they become effector T-cells (By similarity). Negatively regulates RLR-mediated antiviral response by promoting SMURF1-mediated ubiquitination and subsequent degradation of MAVS (PubMed:23087404). Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus where it mediates KCNH2 degradation (PubMed:26363003). In cortical neurons, mediates the ubiquitination of the divalent metal transporter SLC11A2/DMT1 by NEDD4L, leading to its down-regulation and protection of the cells from cobalt and iron toxicity (PubMed:19706893). Important for normal development of dendrites and dendritic spines in cortex (By similarity). Enhances the ubiquitination of BRAT1 mediated by: NEDD4, NEDD4L and ITCH and is required for the nuclear localization of ubiquitinated BRAT1 (PubMed:25631046). Enhances the ITCH-mediated ubiquitination of MAP3K7 by recruiting E2 ubiquitin-conjugating enzyme UBE2L3 to ITCH (By similarity). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (PubMed:20534535). Inhibits cell proliferation by promoting PTEN nuclear localization and changing its signaling specificity (PubMed:25801959).
Gene: NDFIP1
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分子量
- 25 kDa
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基因ID
- 80762
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UniProt
- Q9BT67
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途径
- Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity
抗原
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