Cullin 7 抗体 (C-Term)
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北京 101111
Quick Overview for Cullin 7 抗体 (C-Term) (ABIN6257116)
抗原
See all Cullin 7 (CUL7) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- CUL7 Antibody detects endogenous levels of total CUL7.
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预测反应
- Horse,Sheep,Dog
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human CUL7, corresponding to a region within C-terminal amino acids.
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亚型
- IgG
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anti-Cullin 7 (CUL7) (C-Term) antibody
CUL7 适用: 人 WB, IP, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 7 (CUL7) (AA 312-450) antibodyCUL7 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 7 (CUL7) antibodyCUL7 适用: 人, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 7 (CUL7) (AA 1-100) antibodyCUL7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G11 unconjugated
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应用备注
- WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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有效期
- 12 months
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- Cullin 7 (CUL7)
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别名
- CUL7
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背景
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Description: Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695). Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5 (PubMed:24793696). Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1 (PubMed:21572988, PubMed:24362026). Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation (PubMed:24362026). Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development (PubMed:20139075). Does not promote polyubiquitination and proteasomal degradation of p53/TP53 (PubMed:16547496, PubMed:17332328). While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions.
Gene: CUL7
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分子量
- 191 kDa
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基因ID
- 9820
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UniProt
- Q14999
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途径
- ER-Nucleus Signaling
抗原
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