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CDK1 抗体 (pThr14)

Name: CDK1 抗体 (pThr14)
SKU: ABIN6256473
Availability: OutOfStock

This anti-CDK1 antibody is a 兔多克隆 antibody detecting CDK1 in WB, ELISA, IF 和 ICC. Suitable for 人, 小鼠和大鼠.

Western blot analysis of CDK1/CDC2 phosphorylation expression in Forskolin treated HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (CDK1 抗体 (pThr14))

IF/ICC

ABIN6267447 staining C6 cells by ICC/IF. (CDK1 抗体 (pThr14))

IF/ICC

3 images

产品编号 ABIN6256473

发货至: 中国

Datasheet as PDF

Quick Overview for CDK1 抗体 (pThr14) (ABIN6256473)

抗原

See all CDK1 抗体

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This CDK1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product CDK1 Primary Antibody

抗原表位
34

23

23

20

17

16

9

9

9

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8

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4

4

4

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1

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1
pThr14

特异性

Phospho-CDK1/CDC2 (Thr14) Antibody detects endogenous levels of CDK1/CDC2 only when phosphorylated at Threonine 14.

预测反应

Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14.

亚型

IgG
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 59-182) antibody
CDK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (pTyr15) antibody
CDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibody
CDK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G10 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibody
CDK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A4-1A9 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 208-258) antibody
CDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) antibody
CDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibody
CDK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 8F1 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibody
CDK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 66-297) antibody
CDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 201-297) antibody
CDK1 适用: 人, 小鼠, 大鼠, 犬, 绵羊 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

别名

CDK1

背景

Description: Serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis, regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431).
Gene: CDK2

分子量

34kDa

基因ID

983

UniProt

P06493

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA