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RUNX1 抗体 (pSer304)

Name: RUNX1 抗体 (pSer304)
SKU: ABIN6256258
Availability: OutOfStock

This anti-RUNX1 antibody is a 兔多克隆 antibody detecting RUNX1 in WB, ELISA, IHC, IF 和 ICC. Suitable for 人, 小鼠和大鼠.

Western blot analysis of Phospho-AML1 (Ser303) expression in various lysates (RUNX1 抗体 (pSer304))

IF/ICC

ABIN6267588 at 1/100 staining Rat heart tissue by IHC-P. (RUNX1 抗体 (pSer304))

IHC

Western blot analysis of AML1 phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (RUNX1 抗体 (pSer304))

ABIN6267588 at 1/100 staining human lung tissues sections by IHC-P. (RUNX1 抗体 (pSer304))

IHC

5 images

产品编号 ABIN6256258

发货至: 中国

Quick Overview for RUNX1 抗体 (pSer304) (ABIN6256258)

抗原

See all RUNX1 抗体

RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This RUNX1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product RUNX1 Primary Antibody

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pSer304

特异性

Phospho-RUNX1 / AML1 (Ser304) Antibody detects endogenous levels of RUNX1 / AML1 only when phosphorylated at Serine 304.

预测反应

Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human RUNX1 / AML1 around the phosphorylation site of Ser304.

亚型

IgG
anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 210-310) antibody
RUNX1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2C10 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 150-250) antibody
RUNX1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 221-480) antibody
RUNX1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) antibody
RUNX1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 210-310) antibody
RUNX1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 3A1 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 227-255) antibody
RUNX1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 237-337) antibody
RUNX1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6C12A5 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) antibody
RUNX1 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 5A1 unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 200-233), (Middle Region) antibody
RUNX1 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Runt-Related Transcription Factor 1 (RUNX1) (AA 227-255) antibody
RUNX1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB15210 unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

别名

RUNX1

背景

Description: Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity).
Gene: RUNX1

分子量

50kDa

基因ID

861

UniProt

Q01196