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Vinculin 抗体 (pTyr822)

Name: Vinculin 抗体 (pTyr822)
SKU: ABIN6256040
Availability: OutOfStock

This anti-Vinculin antibody is a 兔多克隆 antibody detecting Vinculin in WB, IHC, ELISA, IF 和 ICC. Suitable for 人, 小鼠和大鼠.

Western blot analysis of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (Vinculin 抗体 (pTyr822))

ABIN6267417 at 1/100 staining human Heart muscle tissue sections by IHC-P. (Vinculin 抗体 (pTyr822))

IHC

ABIN6267417 staining HepG2 cells by ICC/IF. (Vinculin 抗体 (pTyr822))

IF/ICC

3 images

产品编号 ABIN6256040

发货至: 中国

Quick Overview for Vinculin 抗体 (pTyr822) (ABIN6256040)

抗原

See all Vinculin (VCL) 抗体

Vinculin (VCL)

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This Vinculin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

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pTyr822

特异性

Phospho-Vinculin (Tyr822) Antibody detects endogenous levels of Vinculin only when phosphorylated at Tyrosine 822.

预测反应

Pig,Horse,Sheep,Rabbit,Dog,Chicken

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr822.

亚型

IgG
anti-Vinculin (VCL) (AA 1-1066) antibody
VCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C6-1B5 unconjugated

anti-Vinculin (VCL) (AA 879-1064) antibody
VCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vinculin (VCL) (AA 903-937) antibody
VCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55983 unconjugated

anti-Vinculin (VCL) (AA 1020-1134) antibody
VCL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Vinculin (VCL) (AA 634-668) antibody
VCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55982 unconjugated

anti-Vinculin (VCL) (AA 1-1066) antibody
VCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F8-1D4 unconjugated

anti-Vinculin (VCL) (AA 174-322) antibody
VCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2575 unconjugated

anti-Vinculin (VCL) (AA 174-322) antibody
VCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2572 unconjugated

anti-Vinculin (VCL) (AA 767-1066) antibody
VCL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vinculin (VCL) antibody
VCL 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

Vinculin (VCL)

别名

VCL

背景

Description: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Gene: VCL

分子量

123kDa

基因ID

7414

UniProt

P18206

途径

Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2