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Histone H4 抗体 (acLys12)

适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6254899
发货至: 中国
  • 抗原 See all Histone H4 抗体
    Histone H4
    抗原表位
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    acLys12
    适用
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    人, 小鼠, 大鼠
    宿主
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    克隆类型
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    多克隆
    标记
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    This Histone H4 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)
    特异性
    Acetyl-Histone H4 (Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine12.
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    affinity purification
    免疫原
    The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.
    亚型
    IgG
  • 应用备注
    WB: 1:500~1:2000 IHC: 1:50~1:200 IF 1:200
    限制
    仅限研究用
  • 生效 #104615 (Immunohistochemistry)
    '独立验证'标志
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104615
    日期
    2024.09.22
    抗原
    Histone H4
    Lot Number
    lk4553
    Method validated
    Immunohistochemistry
    Positive Control

    CD1 mouse brain fixed in 4% paraformaldehyde.

    Negative Control

    Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.

    Notes
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN6254899
    Secondary Antibody
    Alexa Fluor 488 goat anti-rabbit (Invitrogen by Thermo Fisher Scientific, A11034, lot # 2380031), Alexa Fluor 488 donkey anti-rabbit (Invitrogen by Thermo Fisher Scientific A32790 lot # XJ357262).
    Full Protocol
    • SAMPLE ANALYZED: Old (> 18 months) CD1 mouse brain.
    • Mice were perfused with paraformaldehyde 4% in 0.1M phosphate buffer (PB) pH 7.4 and samples were post-fixed in the same fixative for 2 additional hours at room temperature. After fixation, samples were washed, dehydrated, and embedded in paraffin wax.
    • Following several washes in PBS, the brain was cut with a microtome (6 μm-thick sections) in parasagittal sections mounted on glass slides.
    • After paraffin removal, sections were incubated for 1 hour at room temperature in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot # 00583) to block non-specific binding sites. Sections were then incubated overnight at room temperature with the primary antibody anti-Histone H4 (acLys12) (ABIN6254899) at 1:50, 1:100, and 1:200, in PBS-BSA (Sigma, A7906)-PLL (Sigma, P1524).
    • Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 minutes (95–100°C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, sections were washed (distilled water, 2 times for 5 min each; followed by PBS for 5 min).
    • Sections were washed 3×5 min in 0.01M PBS.
    • Sections were then incubated with the anti-rabbit secondary antibody Alexa Fluor 488, 1:500, in 0.1M PBS, for 1 hour at room temperature.
    • Sections were washed 3×5 min in 0.01M PBS.
    • Specimens were mounted in Fluoroshield (Sigma, F6182, lot # MKCB0153V).
    • Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 20× or 40× magnification.
    Experimental Notes

    Passed. The antibody works for IHC-P at 1:100 and 1:200 concentrations with or without microwave antigen retrieval. Microwave treatment increases the intensity of staining.

  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store at -20 °C.Stable for 12 months from date of receipt
    有效期
    12 months
  • 抗原
    Histone H4
    Abstract
    Histone H4 产品
    背景

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

    分子量
    11kDa
    基因ID
    121, 504, 554
    UniProt
    P62805
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