Histone H4 抗体 (acLys12)
(1 validation)-
- 抗原 See all Histone H4 抗体
- Histone H4
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抗原表位
- acLys12
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone H4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)
- 特异性
- Acetyl-Histone H4 (Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine12.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- affinity purification
- 免疫原
- The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.
- 亚型
- IgG
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anti-Histone H4 (acLys12) antibody
适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (meLys20) antibody适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (2meLys20) antibody适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (3meLys20) antibody适用: 人 WB, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys8) antibody适用: 人 WB, IHC, IF, IP, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys5) antibody适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Histone H4 (acLys16) antibody适用: 人, Saccharomyces cerevisiae, Drosophila melanogaster WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (meLys20) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys16) antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H4 (acLys8) antibody适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:500~1:2000 IHC: 1:50~1:200 IF 1:200
- 限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104615
- 日期
- 2024.09.22
- 抗原
- Histone H4
- Lot Number
- lk4553
- Method validated
- Immunohistochemistry
- Positive Control
CD1 mouse brain fixed in 4% paraformaldehyde.
- Negative Control
Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.
- Notes
- Primary Antibody
- ABIN6254899
- Secondary Antibody
- Alexa Fluor 488 goat anti-rabbit (Invitrogen by Thermo Fisher Scientific, A11034, lot # 2380031), Alexa Fluor 488 donkey anti-rabbit (Invitrogen by Thermo Fisher Scientific A32790 lot # XJ357262).
- Full Protocol
- SAMPLE ANALYZED: Old (> 18 months) CD1 mouse brain.
- Mice were perfused with paraformaldehyde 4% in 0.1M phosphate buffer (PB) pH 7.4 and samples were post-fixed in the same fixative for 2 additional hours at room temperature. After fixation, samples were washed, dehydrated, and embedded in paraffin wax.
- Following several washes in PBS, the brain was cut with a microtome (6 μm-thick sections) in parasagittal sections mounted on glass slides.
- After paraffin removal, sections were incubated for 1 hour at room temperature in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot # 00583) to block non-specific binding sites. Sections were then incubated overnight at room temperature with the primary antibody anti-Histone H4 (acLys12) (ABIN6254899) at 1:50, 1:100, and 1:200, in PBS-BSA (Sigma, A7906)-PLL (Sigma, P1524).
- Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 minutes (95–100°C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, sections were washed (distilled water, 2 times for 5 min each; followed by PBS for 5 min).
- Sections were washed 3×5 min in 0.01M PBS.
- Sections were then incubated with the anti-rabbit secondary antibody Alexa Fluor 488, 1:500, in 0.1M PBS, for 1 hour at room temperature.
- Sections were washed 3×5 min in 0.01M PBS.
- Specimens were mounted in Fluoroshield (Sigma, F6182, lot # MKCB0153V).
- Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 20× or 40× magnification.
- Experimental Notes
Passed. The antibody works for IHC-P at 1:100 and 1:200 concentrations with or without microwave antigen retrieval. Microwave treatment increases the intensity of staining.
生效 #104615 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Histone H4 (acLys12) positive nuclei in the mouse brain. A: Immunoreactive nuclei in the granular layer of the cerebral cortex (original magnification 20x). Microwave antigen retrieval. B: Immunoreactive nuclei in the hippocampus. Note the intense staining]()
Histone H4 (acLys12) positive nuclei in the mouse brain. A: Immunoreactive nuclei in the granular layer of the cerebral cortex (original magnification 20x). Microwave antigen retrieval. B: Immunoreactive nuclei in the hippocampus. Note the intense staining
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
- 有效期
- 12 months
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- 抗原
- Histone H4
- Abstract
- Histone H4 产品
- 背景
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Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene: HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4
- 分子量
- 11kDa
- 基因ID
- 121, 504, 554
- UniProt
- P62805
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