Abscisic Acid Insensitive 5 (ABI5) 抗体
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北京 101111
Quick Overview for Abscisic Acid Insensitive 5 (ABI5) 抗体 (ABIN4966067)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-ABI5 | Abscisic acid insensitive 5 (peptide antibody)
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Brassica napus,Populus trichocarpa
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence
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应用备注
- 1: 140 (IL), 1 : 200 (WB)
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说明
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ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as most sensitive detection reagents.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water to each tube
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Abscisic Acid Insensitive 5 (ABI5)
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背景
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Background: ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.
Additional information: MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here.
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分子量
- 47 kDa
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基因ID
- 818199
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UniProt
- Q9SJN0
抗原
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