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FTO 抗体 (N-Term)

Cited in 6+ publications. This 小鼠 单克隆 anti-FTO antibody (Clone 5-2H10) specifically detects FTO in WB, ELISA 和 ICC. The antibody is reactive with 大鼠 samples.
产品编号 ABIN6254099
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for FTO 抗体 (N-Term) (ABIN6254099)

抗原

See all FTO 抗体
FTO (Fat Mass and Obesity-Associated (FTO))

适用

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大鼠

宿主

  • 79
  • 9
  • 1
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小鼠

克隆类型

  • 47
  • 43
单克隆

标记

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This FTO antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

克隆位点

5-2H10
  • 抗原表位

    • 15
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    • 1
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    N-Term

    特异性

    Specific for endogenous levels of the ~58 kDa FTO protein.

    交叉反应

    人, 小鼠, 大鼠

    预测反应

    bovine, canine, horse, non-human primate, sheep

    产品特性

    Our Anti-FTO (Fat mass and obesity related protein) Antibody primary antibody is mouse monoclonal. It detects FTO (Fat mass and obesity related protein) and is protein g purified. It is great for use in ELISA, WB, ICC.

    纯化方法

    Protein G purified culture supernatant.

    免疫原

    Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat FTO, conjugated to keyhole limpet hemocyanin (KLH).

    亚型

    IgG2a
  • 应用备注

    Dilution Range: ICC: 1:100-1:600

    Dilution Range: WB: 1:1000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

    储存条件

    -20 °C

    储存方法

    Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

    有效期

    12 months
  • Mao, Dong, Liu, Guo, Ma, Shen, Qian: "m6A in mRNA coding regions promotes translation via the RNA helicase-containing YTHDC2." in: Nature communications, Vol. 10, Issue 1, pp. 5332, (2020) (PubMed).

    Liu, Zhou, Mao, Ji, Qian: "Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates." in: Nature chemical biology, Vol. 15, Issue 9, pp. 865-871, (2019) (PubMed).

    Zhou, Wan, Shu, Mao, Liu, Yuan, Zhang, Hess, Brüning, Qian: "N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response." in: Molecular cell, Vol. 69, Issue 4, pp. 636-647.e7, (2019) (PubMed).

    Wang, Li, Yue, Wang, Kumar, Wechsler-Reya, Zhang, Ogawa, Kellis, Duester, Zhao: "N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications." in: Nature neuroscience, Vol. 21, Issue 2, pp. 195-206, (2019) (PubMed).

    Yu, Chen, Huang, Zhu, Song, Zhu, Park, Ji: "Dynamic m6A modification regulates local translation of mRNA in axons." in: Nucleic acids research, Vol. 46, Issue 3, pp. 1412-1423, (2019) (PubMed).

    Zhou, Wan, Gao, Zhang, Jaffrey, Qian: "Dynamic m(6)A mRNA methylation directs translational control of heat shock response." in: Nature, Vol. 526, Issue 7574, pp. 591-4, (2015) (PubMed).

  • 抗原

    FTO (Fat Mass and Obesity-Associated (FTO))

    别名

    FTO

    背景

    Synonyms: AlkB homolog 9 antibody, ALKBH9 antibody, Alpha-ketoglutarate-dependent dioxygenase FTO antibody, AW743446 antibody, Fat mass and obesity-associated protein antibody, FATSO, MOUSE, HOMOLOG OF antibody, Fto antibody, FTO_HUMAN antibody, GDFD antibody, KIAA1752 antibody, mKIAA1752 antibody, Protein fatso antibody

    Description Logic: Our Anti-FTO (Fat mass and obesity related protein) Antibody primary antibody is mouse monoclonal. It detects FTO (Fat mass and obesity related protein) and is protein g purified. It is great for use in ELISA, WB, ICC.

    Gene Name Alternatives: FTO

    分子量

    58 kDa

    基因ID

    79068

    UniProt

    Q9C0B1
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