ACTH 抗体 (AA 25-39)
Quick Overview for ACTH 抗体 (AA 25-39) (ABIN6252886)
抗原
See all ACTH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 25-39
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone were used as the immunogen for this ACTH antibody.
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亚型
- IgG1
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anti-Adrenocorticotropic hormone (ACTH) antibody
ACTH 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3A4H4 unconjugated
anti-Adrenocorticotropic hormone (ACTH) antibodyACTH 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 1A9C6 unconjugated
anti-Adrenocorticotropic hormone (ACTH) (AA 1-39) antibodyACTH 适用: 人, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Adrenocorticotropic hormone (ACTH) (AA 18-39) antibodyACTH 适用: 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Adrenocorticotropic hormone (ACTH) antibodyACTH 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: order BSA free format for coating
Flow Cytometry: 0.5-1 μg/10^6 cells
IF: 1-2 μg/mL
IHC (FFPE):0.5-1 μg/mL
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in 10 mM Citrate Buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- ACTH (Adrenocorticotropic hormone (ACTH))
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别名
- ACTH
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物质类
- Hormone
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背景
- ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
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基因ID
- 5443
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UniProt
- P01189
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
抗原
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